ABSTRACT
A retrospective analysis was performed in two major HIV/AIDS referral hospitals in Beijing to evaluate the prevalence of Mycobacterium tuberculosis (MTB) and non-tuberculous mycobacterial (NTM) infections in HIV-infected patients. A total of 627 patients' data were reviewed, and 102 (16.3%) patients were diagnosed with culture-confirmed mycobacterial infection, including 84 with MTB, 16 with NTM, and 2 with both MTB and NTM. The most frequent clinical complication by mycobacterial infection was pulmonary infection (48/102, 47.1%). The overall rates of multidrug-resistant TB (MDR-TB) and extensively drug-resistant TB (XDR-TB) were 11.9% and 3.4%, respectively. This study underlines the urgent need to intensify screening for mycobacteria coinfection with HIV and to prevent the spread of drug-resistant TB among HIV-infected patients.
Subject(s)
Adult , Female , Humans , Male , AIDS-Related Opportunistic Infections , Epidemiology , Microbiology , Beijing , Coinfection , Extensively Drug-Resistant Tuberculosis , Epidemiology , Microbiology , HIV Infections , Epidemiology , Microbiology , Hospitals, Urban , Mycobacterium Infections, Nontuberculous , Epidemiology , Microbiology , Mycobacterium tuberculosis , Nontuberculous Mycobacteria , Prevalence , Retrospective Studies , Sputum , Microbiology , Tuberculosis, Multidrug-Resistant , Epidemiology , Microbiology , Tuberculosis, Pulmonary , Epidemiology , MicrobiologyABSTRACT
70 clinical Mycobacterium tuberculosis strains isolated from AIDS patients in two HIV/AIDS referral hospitals in Beijing were used in this study. M. tuberculosis and non-tuberculosis mycobacterium (NTM) were identified by using multi-locus PCR. M. tuberculosis was genotyped by using 15-locus MIRU-VNTR technique and spoligotyping afterwards. Meanwhile, the drug susceptibilities of the strains to the four first-line anti TB drugs (rifampin, isoniazid, streptomycin, and ethambutol) and the four second-line anti-TB drugs (capreomycin, kanamycin, ofloxacin, and ethionanide) were tested with proportional method. In this study, M. tuberculosis and NTM strains isolated from AIDS patients with TB-like symptoms were identified and genotyping analysis indicated that Beijing genotype was the predominant genotype. In addition, the prevalence of drug-resistant TB, especially the prevalence of XDR-TB, was higher than that in TB patients without HIV infection.
Subject(s)
Humans , AIDS-Related Opportunistic Infections , Microbiology , Antitubercular Agents , Pharmacology , China , Microbial Sensitivity Tests , Mycobacterium tuberculosis , Classification , Phylogeny , Tuberculosis , MicrobiologyABSTRACT
<p><b>OBJECTIVE</b>To investigate the SCCmec genotyping, subtype and antimicrobial susceptibility tests in methicillin resistant staphylococcus aureus to guide the clinical treatment and provide the proof for molecular epidemiology.</p><p><b>METHODS</b>To detect mecA gene and SCCmec genetyping and subtype in 50 MRSA by PCR. According to CLSI's guideline, antimicrobial susceptibility tests were performed with disk diffusion.</p><p><b>RESULTS</b>All 50 MRSA had mecA genes. 45 strains were SCCmec III types; 3 strains were SCCmec III A types; 2 strains were SCCmec II types. There were no SCCmec I and SCCmec IV types. SCCmec II, SCCmec III and SCCmec III A type strains were all multiresistant.</p><p><b>CONCLUSION</b>50 MRSA are all multiresistant. SCCmec III are the main types.</p>
Subject(s)
Humans , Anti-Bacterial Agents , Pharmacology , Bacterial Proteins , Genetics , China , Epidemiology , Drug Resistance, Multiple, Bacterial , Genotype , Methicillin-Resistant Staphylococcus aureus , Classification , Genetics , Microbial Sensitivity Tests , Penicillin-Binding Proteins , Phylogeny , Staphylococcal Infections , Epidemiology , MicrobiologyABSTRACT
<p><b>OBJECTIVE</b>To compare the liver function index and clinical characters in 122 patients with acute hepatitis E virus overlapping with other infection.</p><p><b>METHODS</b>The liver function index and clinical characters of 122 patients with acute hepatitis E virus overlapping infection and 40 patients with acute hepatitis E were retrospectively analyzed.</p><p><b>RESULTS</b>No significant differences of ALT, AST, TBIL, DBIL were found between acute hepatitis E groups and overlapping infection hepatitis A or hepatitis B (P > 0.05). However, there were significant differences of Albumin (ALB) and Globulin (GLO) were found between acute hepatitis E groups and overlapping infection hepatitis B (P < 0.01). In acute hepatitis E overlapping infected hepatitis B or hepatitis A patients, more and severe complications were also observed.</p><p><b>CONCLUSION</b>The patients with acute Hepatitis E virus, especially Hepatitis E virus overlapping infection, need to pay more clinical monitor, prevent complication early and lower death rates.</p>
Subject(s)
Adolescent , Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Young Adult , Acute Disease , Albumins , Metabolism , Globulins , Metabolism , Hepatitis A , Diagnosis , Metabolism , Hepatitis B , Diagnosis , Metabolism , Hepatitis E , Diagnosis , Metabolism , Liver , Metabolism , Liver Function Tests , Retrospective StudiesABSTRACT
512?g/mL; MIC of 16 VIE to vancomycin were 16?g/mL; MIC of 8 VIE to vancomycin were 8?g/mL. It is a risk factor for hospital infection that VRE carriage of inpatients in intestinal tract is high. There is 100% agreement be-tween phenotypes and genotypes in 46 vancomycin resistance enterococci. VRE are multiresistant. Part iso-lates have high homology.